摘要：【目的】研究茅尾海無瓣海桑根际土壤细菌多样性及抑菌活性，挖掘更多潜在新物种和高效活性物质，为进一步研究红树植物根际土壤中细菌的次生代谢产物及研发新型高效生物农药提供参考依据。【方法】从广西钦州茅尾海红树林保护区采集无瓣海桑根际土壤，采用6种分离培养基分离可培养细菌；通过16S rRNA基因序列的系统发育学分析其物种多样性，并采用牛津杯法考察根际细菌对甘蔗鞭黑粉菌的抑制活性。【结果】从无瓣海桑根际中分离得到可培养细菌97株，通过16S rRNA序列比对排重后，从中获得57株不同细菌，可归为4门5纲9目15科21属，有1株Cellvibrio sp.的潜在新种。其中29株细菌能够抑制甘蔗鞭黑粉菌活性，分别有12、14和14株细菌对甘蔗鞭黑粉菌菌丝生长、“+”担孢子增殖和“-”担孢子增殖表现出抑制活性，抑菌圈直径范围为7.54～21.81 mm，其中有9株细菌能够抑制2～3种甘蔗鞭黑粉菌的形态。【结论】广西茅尾海红树林保护区中无瓣海桑根际细菌具有较高的物种多样性，蕴藏着潜在新物种资源，且富含抑菌活性菌株。
关键词： 无瓣海桑根际；细菌多样性；甘蔗鞭黑粉菌；抑菌活性；茅尾海红树林
中图分类号： S482.292；Q939.1 文献标志码：A 文章编号：2095-1191（2018）06-1095-07
Abstract：【Objective】Diversity and inhibition activity of rhizospheric bacteria which came from Sonneratia apetala in Maowei Sea were studied to find out more potential new species and high-efficiency active materials， and provided re-ference for the further research on secondary metabolites of the rhizospheric bacteria of mangrove plant and the further development of new and efficient biological pesticides. 【Method】The rhizosphere soil of S. apetala were collected from Maowei Sea mangrove reserve in Qinzhou， Guangxi. Six different media were employed to isolate the cultured bacteria. The species diversity was revealed through phylogenetic analysis of the 16S rRNA gene sequence， and Oxford cup method was used to investigate the inhibitory activity of the rhizosphere bacteria against Sporisorium scitamineum. 【Result】The 97 strains of culturable bacteria were isolated from the rhizosphere of S. apetala.After comparing the 16S rRNA sequence alignments， 57 different strains of bacteria were obtained， which could be classified into 21 genera， 15 families， 9 orders， 5 classes and 4 phyla. There was also a potential new species of Cellvibrio sp. The 29 strains could inhibit the activity of S. scitamineum. That there were 12， 14 and 14 strains of bacteria exhibited inhibitory activity against mycelium growth， proliferation of “+” spores， and proliferation of “-” spores of S. scitamineum respectively. The diameter of inhibition zone was between 7.54 and 21.81，and 9 strains of which could inhibit the morphology of 2 to 3 species of S. scitamineum. 【Conclusion】The rhizospheric bacteria of S. apetala which come from Maowei Sea mangrove reserve in Qinzhou， Guangxi have high species diversity， contain potential new species resources， and are rich in antibacterial activity strains.
Key words： Sonneratia apetala； bacterial diversity； Sporisorium scitamineum； antibacterial activity；Maowei Sea mangrove
相关热词搜索： 细菌 土壤 活性 性及 抑菌
