microRNA在皮肤黑色素瘤血清与毛发中的差异表达

对照组。采用qRT-PCR技术检测两组患者的血清和毛发中miRNA表达情况。将检测结果一致的候选miRNA确定为有意义的共同差异表达生物学指标，利用组间差异倍数筛选出差异>2.5倍差异表达的miRNA。 结果 CM组患者血清中7个miRNA有2个上调，5个下调，上调的miRNA包括miR-4487和miR-4706，下调的miRNA包括miR-16、miR-211、miR-4731、miR-509-3p和miR-514a;毛发中有3个上调，4个下调，上调的miRNA包括miR-211、miR-4487和miR-4731;下调的miRNA包括miR-16、miR-4706、miR-509-3p和miR-514a。与对照组比较，CM组miR-4487在血清和毛发中共同上调，但差异无统计学意义（P > 0.05）。与对照组比较，CM组血清和毛发中has-miR-16和has-miR-509-3p表达显著下调，差异均有统计学意义（P < 0.05），并且一致性较高;与对照组比较，CM组血清和毛发中miR-541a表达虽然显示共同下调，但差异无统计学意义（P > 0.05）。血清和毛发差异表达2.5倍以上的miRNA为miR-16和miR-514a;单独毛发差异表达2.5倍以上的miRNA为miR-4706;单独血清标本4倍以上差异表达为miR-4731;血清标本10倍以上差异表达的miRNA为miR-211和miR-509-3p。 结论 与非肿瘤疾病患者比较，血清和毛发中存在多种miRNA的差异表达，CM患者血清中miR-211和miR-509-3p存在高度显著差异表达，可以作为该肿瘤的生物学标志物;毛发标本miR-4706的差异表达有可能成为黑色素瘤早期非创伤性检测的生物学指标。miRNA检测对CM是可利用的生物学指标，也可能成为今后靶基因治疗的手段。

[关键词] 皮肤黑色素瘤;微小RNA;信号转导通路;差异表达

[中图分类号] R739.5          [文献标识码] A          [文章编号] 1673-7210（2019）09（b）-0007-06

Differential expression of microRNAs in serum and hairs different position of cutaneous melanoma

LIN Qianli   ZHANG Wenjun   WANG Zhongzhi   WANG Hui   JIANG Hua

Department of Plastic Surgery， Shanghai Changzheng Hospital， Navy Military Medical University， Shanghai   200003， China

[Abstract] Objective To investigate the differential expression of microRNAs in cutaneous malignance melanoma in order to provide a basis for the diagnosis and targeted treatment of this malignancy; to screen seven specific microRNAs in serum and hairs of cutaneous malignancy melanoma. Methods From March 2016 to October 2017， 12 cases of cutaneous melanoma （CM）  confirmed by routine histopathology and immunohistochemistry in Shanghai Changzheng Hospital， Navy Military Medical University， were collected as CM group， while 17 cases of non-neoplastic skin diseases were collected as control group. The expression situation of microRNAs in serum and hair of two groups were detected by qRT-PCR. Candidate microRNAs with consistent results were identified as significant common differential expression biological indicators. The differentially expressed microRNAs （> 2.5 times） were screened by the multiple of differences between groups. Results There were 2 up-regulated and 5 down-regulated microRNAs in serum among 7 microRNAs of patients in CM group. The up-regulated microRNAs included miR-4487 and miR-4706， the down-regulated microRNAs included miR-16， miR-211， miR-4731， miR-509-3p and miR-514a. There were 3 up-regulated and 4 down-regulated microRNAs in hairs， the up-regulated microRNAs included miR-211， miR-4487 and miR-4731; the down-regulated microRNAs included miR-16， miR-4706， miR-509-3p and miR-514a. The co-up-regulated microRNAs in serum and hairs of the CM group were microRNAs-4487， but there was no statistically significant difference between the two groups （P > 0.05）. Compared with the control group， the expressions of has-microRNA-16 and has-microRNA-509-3p were significantly down-regulated， the differences were statistically significant （P < 0.05）， and with high consistency. Compared with control group， the expression of miRNA-541a in serum and hairs of CM group was down-regulated， but there was no statistically significant difference （P > 0.05）. MiRNAs with more than 2.5 times differential expression in serum and hairs were miR-16 and miR-514a. In hair alone， miR-4706 was with differential expression of more than 2.5 times. The miR-4731 was more than 4 times differentially expressed in serum samples alone. MiRNAs with more than 10 times differential expression in serum were miR-211 and miR-509-3p. Conclusion Compared with non-cancer patients， there are many differentially expressed microRNAs in serum and hairs. There are highly significant differentially expressed microRNAs 211 and 509-3p in serum of CM patients， which can be used as biological markers of the tumor. Differential expression of microRNAs 4706 in hair samples may be the biology of early noninvasive detection of melanoma. Indicators. Detection of microRNAs is an available biological indicator for CM， and may become a means of target gene therapy in the future.